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Assay-ready cells are pre-plated, adherent cell lines that have been grown, plated and cryopreserved in microplates. They are conveniently stored at -80°C and ready-to-use 24 hours after thawing at the appropriate cell density without cell culture, counting, or validation steps.
The manufacturing process of cell plates at CryoLogyx is powered Cryoshield™ technology. This technology involves the use of specialized macromolecular cryoprotectant. These cryoprotectants are large molecules similar to anti-freeze proteins, closely mimicking the structure and function of biomacromolecules used by extremophiles, organisms that survive in extreme environments. This advanced suite of cryoprotectant materials allows for very high cell recoveries and enables us to cryopreserve cells in various formats.
ToxReady™ Models:
PlaqueReady™ Models:
Our plates can be provided at three confluency levels, i.e. cell surface coverage.
Low confluency: 20 - 30%
Medium confluency: 40 - 60%
High confluency: over 70%
Detailed instructions can be found in the Protocols Page.
Store in a -80 °C freezer. Use by expiry date on the product packaging.
Yes, we specialize in providing customized assay-ready cell plates to precisely align with your specific commercial and technical requirements. Our technology enables us to meticulously prepare cell plates that are optimized for various assays, ensuring seamless integration into your research and development processes. Please contact us at info@cryologyx.com with your request.
We will send your plates with dry ice and standard next day shipping in mainland UK if you order before 10am (BST).
Please find the expiry date on the packaging.
The plates offered by CryoLogyx are indeed compatible with high-throughput screening. ToxReady™ plates are compatible with all major drug toxicity and interaction studies. They are well-suited for various types of high-throughput screening applications in drug discovery and toxicology. The cells are provided in standard plate formats, which means that existing procedures do not need to be modified, further facilitating ease of use in high-throughput settings.
Yes, we offer dedicated technical support to assist with any troubleshooting, ensuring that you can confidently rely on our products for consistent results. Please contact us at info@cryologyx.com for any technical queries.
Navigate to Store Page to browse our catalogue. Select the products you need, add them to your cart, and proceed to checkout.
We accept Stripe payment or, alternatively, please contact us at info@cryologyx.com to request a quote.
Yes, each batch of plates is tested mycoplasma.
Yes, our Cryoshield™ formulation, which is used to manufacture assay-ready plates, contains 10% FBS and 10% DMSO. However, we aim to remove all animal-based products from our formulations by Q3 2024.
Contact us at info@cryologyx.com with your request.
These are the most asked questions from our customers.
Although we advise 12 minutes, thawing time can vary based on conditions and dimensions of the incubator. A thawing time between 10 - 20 minutes is expected. If this is your first time thawing a plate, start with 12 minutes incubation and check periodically every 2 minutes until no ice is present at the bottom of the well plates.
We recommend thawing the plates immediately after taking them out of the freezer.
The quantity of plates thawed depends on whether the plates are thawed manually or using (semi-)automated systems. If using pipettes, we recommend no more than 3 plates at once. However, more plates can be thawed using faster setups. As a golden rule, you can thaw as many plates within 10 minutes.
The thawing media recommended on the general protocols is also the cell culture media recommended by either ATCC or ECACC. Alternative media can be used, however, post-thaw quality cannot be guaranteed as we haven't validate it. Customers that have used alternative media have not reported have not reported any differences in expected quality.
We perform quality control procedures to ensure that the correct confluency is expected post-thaw. However, if the confluency is less than expected, the plates can be left for additional 24-48 hours in the incubator to enable cell proliferation.