CryoShield™ 10 100ml Freezing Media
CryoShield™ 10 is an advanced cryoprotectant designed to shield cells from freezing and thawing damage. Formulated with 5% DMSO and CryoLogyx’s patented CryoShield™ ingredient, it forms an extracellular protective matrix that significantly reduces cryoinjury. With a simple medium exchange after thawing, CryoShield™ 10 is easily removed, ensuring minimal interference with downstream applications.
Content:
CryoShield™
10% DMSO
Buffered solution
Key benefits:
Minimises Cryoinjury
Extracellular Protection
Easy Removal
Validated for Wide Range of Cell Types
Reliable and Consistent
CryoShield™ 10 is an advanced cryoprotectant designed to shield cells from freezing and thawing damage. Formulated with 5% DMSO and CryoLogyx’s patented CryoShield™ ingredient, it forms an extracellular protective matrix that significantly reduces cryoinjury. With a simple medium exchange after thawing, CryoShield™ 10 is easily removed, ensuring minimal interference with downstream applications.
Content:
CryoShield™
10% DMSO
Buffered solution
Key benefits:
Minimises Cryoinjury
Extracellular Protection
Easy Removal
Validated for Wide Range of Cell Types
Reliable and Consistent
CryoShield™ 10 is an advanced cryoprotectant designed to shield cells from freezing and thawing damage. Formulated with 5% DMSO and CryoLogyx’s patented CryoShield™ ingredient, it forms an extracellular protective matrix that significantly reduces cryoinjury. With a simple medium exchange after thawing, CryoShield™ 10 is easily removed, ensuring minimal interference with downstream applications.
Content:
CryoShield™
10% DMSO
Buffered solution
Key benefits:
Minimises Cryoinjury
Extracellular Protection
Easy Removal
Validated for Wide Range of Cell Types
Reliable and Consistent
Spheroid Cryopreservation
Spheroids frozen with CryoShield™ 10 demonstrated minimal membrane damage and a significantly higher proportion of viable cells compared to the 10% DMSO 24 h after thawing.
luorescent images of HepG2 spheroids showing from left: non-frozen control, samples frozen with 10% DMSO, and Assay Ready CryoShield™ Spheroids. Viable cells, exhibiting intact membranes, were labelled green with calcein-AM. Dead cells, displaying membrane damage, were labeled red with EthD-III. Below, Hoechst 33342 staining staining the nuclei of membrane-intact cells. On the right quantification of proportion of viable cells by ATP content in spheroids 24 hours post-thaw.
Bissoyi et. al. 2023 from the CSO & Co-Founder, Matt Gibson Group